Answer :
Answer:
a. electrogenic
Explanation:
All Class V ATPases carry only H + ions. These proton pumps, present in the membranes of lysosomes, endosomes and plant vacuoles, function to acidify the light of these organs. The pH of lysosomal light can be accurately measured in living cells by using particles labeled with a pH sensitive fluorescent marker. After these particles are phagocytosed by the cellulose and transferred to the lysosomes, the lysosomal pH can be calculated from the spectrum of the emitted fluorescence. The maintenance of the proton gradient 100 or more times between lysosomal light and the cytosol depends on the production of ATP by the cell.
The proton pump driven by ATP of the lysosome and vacuole membranes have been isolated, purified and incorporated into liposomes.
By themselves, proton pumps driven by ATP cannot acidify the light of an article (or extracellular space) because these pumps are electrogenic, that is, a net movement of electric charge takes place during transport.
The pumping of just a few protons causes the accumulation of positively charged H + ions on the exoplasmic (inner) face of the membrane of the organ. For each H + pumped from one side to the other, a negative ion (eg OH- or Cl-) will be "left behind" on the cytosolic face, causing there a buildup of negatively charged ions. These ions with opposite charge attract each other on opposite faces of the membrane and generate a separation of charges or electrical potential across the membrane.