Answer :
Answer:
he uses two denaturing agents to break down the structure of t a protein
Explanation:
Urea and mercaptoeathanol, he uses urea to break the non-covalent bonds such as hydrogen bonds and ionic bonds that hold together the secondary structure of the protein as well as parts of the tertiary structure and used mercaptoethanol to break the covalent bonds that hold the tertiary structure of the protein.
Cristian studied a particular type of enzyme called ribonuclease, this protein consisted of 124 amino acids in its primary sequence and four disulfide bonds in the tertiary structure.
The finality of this experiment was to see under what conditions after using the agents that ribonuclease reforms.
